Understanding the beetles’ ability to survive these intense internal explosions may help in designing blast-protection systems this study shows how the sophisticated and specialized biological design of the system works to simultaneously achieve defensive and protective functions, Ortiz says. The pulsing nature of the spray may help protect the structure of the beetle’s reaction chamber, Arndt says, allowing time for the chamber walls to cool a bit before the next pulse. Both the speed and the heat serve to make the spray even more effective against potential predators, Arndt says. The explosive mechanism used by the bombardier beetle generates a spray that is not only much hotter than that emitted by other insects that use the same chemical irritant, but also propels the jet five times faster. This all takes place so rapidly - not to mention inside the insect - that the process had never been directly observed. Then, after the pressure is released when the liquid is ejected, the membrane relaxes back to its original state and the passage reopens, allowing the next pulse to form. The opening and closing of this passageway between a chamber holding the precursor liquid and an explosion chamber seems to take place passively an increase in pressure during the explosion expands the membrane, closing the valve. They show that spray pulsation is controlled by the passageway between two internal chambers two structures control this process: a flexible membrane and a valve. The X-ray images of the explosion reveal the dynamics of vapor inside the beetles’ abdomens. They used a facility at Argonne National Laboratory to carry out the experiments and produce detailed images that revealed, for the first time, how the process works, with a camera recording the action at a rate of 2,000 frames per second. In the current study, the researchers used high-speed synchrotron X-ray imaging to “see” inside the abdomens of living bombardier beetles during explosions. “For decades, the complex mechanism of how the bombardier beetle achieves spray pulsation as a chemical defense has not been understood, because only external observations were used previously,” Ortiz says. As the materials combine to form the irritant, they also give off intense heat that brings the liquid almost to the boiling point - and, in the process, generates the pressure needed to expel it in a jet. The key is that they synthesize the chemical at the instant of use, mixing two chemical precursors in a protective chamber in their hindquarters. But bombardier beetles are unique in their ability to superheat the liquid and expel it in an intense, pulsating jet. The liquid these beetles eject is called benzoquinone, and is actually a fairly common defensive agent among insects, Arndt says. “Their defensive mechanism is highly effective,” Arndt says, making bombardier beetles “invulnerable to most vertebrates, and invertebrates” - except for a few very specialized predators that have developed countermeasures against the noxious spray.

The findings are published this week in the journal Science by MIT graduate student Eric Arndt, professor of materials science and engineering Christine Ortiz, Wah-Keat Lee of Brookhaven National Laboratory, and Wendy Moore of the University of Arizona. But now that conundrum has been solved, thanks to research by a team at MIT, the University of Arizona, and Brookhaven National Laboratory. Researchers had been baffled by the half-inch beetles’ ability to produce this noxious spray while avoiding any physical damage. Virtually no other animals prey on them, because of one particularly effective defense mechanism: When disturbed or attacked, the beetles produce an internal chemical explosion in their abdomen and then expel a jet of boiling, irritating liquid toward their attackers. Bombardier beetles, which exist on every continent except Antarctica, have a pretty easy life.

Bombardier beetle grounded